Affiliation:
1. Student Research Committee, School of Medicine Guilan University of Medical Sciences Rasht Iran
2. Farhikhtegan Medical Convergence Sciences Research Center,Farhikhtegan Hospital Tehran Medical Sciences Islamic AzadUniversity Tehran Iran
3. Burn and Regenerative Medicine Research Center Guilan University of Medical Sciences Rasht Iran
4. Department of Microbiology, School of Medicine Guilan University of Medical Sciences Rasht Iran
5. Department of Biostatistics and Epidemiology, School of Health Guilan University of Medical Sciences Rasht Iran
Abstract
AbstractThe present study investigated the antimicrobial and anti‐biofilm effects of indigenous Lactobacillus probiotic strains on Pseudomonas aeruginosa isolated from burn wound infection in laboratory conditions. The effect of 7 probiotic strains isolated from infant faeces on the pathogenicity factors of P. aeruginosa, including protease, elastase, antibiofilm and antipyocyanin was measured. Also, diffusion methods in the well and micro broth dilution were used to evaluate the antimicrobial activity of probiotics. All tests were performed in triplicate. A negative control and a positive control were used for each test. SPSS version 22 software was used for statistical analysis, and a p < 0.05 was considered statistically significant. A total of 30 clinical isolates of P. aeruginosa were isolated. The elastolytic activity of P. aeruginosa isolates decreased after adding Cell free supernatant (CFS) of each Lactobacillus. L1, L4, L5, and L6 strains had a 100% inhibitory effect on pathogen isolates. L3 and L7 strains had the lowest inhibitory effect. The inhibitory effect of CFS extracted from lactobacilli on protease production by P. aeruginosa. L1, L4, L5, and L6 strains had an inhibitory effect on all tested isolates. L2, L3, and L7 strains had a less inhibitory effect. L4 strain had the highest inhibitory effect on pyocyanin production by P. aeruginosa (50%), followed by L5 (43.3%), L1 (40%), and L6 (23.3%) strains. L3 and L7 strains had no inhibitory effect on the pyocyanin production of P. aeruginosa isolates. It was found that the CFS of 4 isolates (L1, L4, L5, and L6) was the most active extract and had a 100% inhibitory effect against biofilm formation of all P. aeruginosa strains. The L3 strain had the least inhibitory effect against the biofilm formation of pathogens. Overall, this study showed that probiotics could be promising alternatives to combat the pathogenicity of P. aeruginosa in burn wounds.
Reference88 articles.
1. A systematic review of self-esteem and related factors among burns patients
2. Evaluating the knowledge and attitudes of the members of the medical community mobilization on first aid for burn injuries in Guilan, Iran;Mobayen M;J Mazandaran Univ Med Sci,2020
3. Effect of early grafting on improvement of lethal area index (la50) in burn patients: a 7‐year investigation in a burn referral Centre in the north of Iran;Mobayen M;Ann Burns Fire Disasters,2017
4. Mortality and Morbidity of Fireworks-Related Burns on the Annual Last Wednesday of the Year Festival (Charshanbeh Soori) in Iran: An 11-Year Study
5. The importance of considering biomechanical properties in skin graft: Are we missing something?