Synergistic Antibioiflm Activity of Probiotic Lactobacillus Acidophilus and Punica Granatum L., Against Pseudomonas Aeruginosa Biofilm

Abstract

Abstract BACKGROUND: Antibiotic resistance is one of the most urgent public health concerns, and multidrug-resistant bacteria are becoming increasingly difficult to treat. Biofilm formation causes continuous infections associated with a variety of medical implants. It is well linked with chronic wounds, chronic obstructive pulmonary disease, urinary tract infections, and cystic fibrosis. There are unquestionable infectious strains of Pseudomonas aeruginosa that cause significant fatality. Several studies have examined the inhibitory antibiofilm effects of natural products on the formation and development of bacterial biofilms, suggesting their potential as alternative agents for bacterial infections. Our goal was to assess the biofilm activity of P. aeruginosa and the individual and combined anti-biofilm forming activity of probiotic Lactobacillus acidophilus and Pomegranate peel extract Punica granatum L., against P. aeruginosa. ATCC culture (4356) of Lactobacillus acidophilus and pomegranate peel extract was utilized to evaluate anti-biofilm activity. RESULTS: The anti-biofilm forming activity of pomegranate peels extract against P. aeruginosa was 29.26±19.09 whereas the anti-biofilm forming activity of Lactobacillus acidophilus against P. aeruginosa was 0.5×106. When used in combination, there was significant synergistic activity between Punica granatum L. (pomegranate peel extract) and Lactobacillus acidophilus. CONCLUSION Pomegranate peel extract and probiotic Lactobacillus acidophilus have antibacterial and antibiofilm properties. Moreover, this unique synergistic mixture of natural product extracts and probiotics has demonstrated more efficiency against rapidly evolving pathogens, serving as promising candidates for developing biofilm inhibitors and perhaps proving as possible environmentally friendly agents against bacteria that produce antibiotic-resistant biofilms.