Enzyme‐based kinetic modelling of ASC–GSH cycle during tomato fruit development reveals the importance of reducing power and ROS availability

Author:

Decros Guillaume1ORCID,Dussarrat Thomas1ORCID,Baldet Pierre1ORCID,Cassan Cédric12ORCID,Cabasson Cécile12ORCID,Dieuaide‐Noubhani Martine1ORCID,Destailleur Alice1,Flandin Amélie12,Prigent Sylvain12ORCID,Mori Kentaro1ORCID,Colombié Sophie12ORCID,Jorly Joana1,Gibon Yves12ORCID,Beauvoit Bertrand1ORCID,Pétriacq Pierre12ORCID

Affiliation:

1. INRAE, UMR1332 BFP University of Bordeaux Villenave d'Ornon 33882 France

2. Bordeaux Metabolome MetaboHUB, PHENOME‐EMPHASIS Villenave d'Ornon 33140 France

Abstract

Summary The ascorbate–glutathione (ASC–GSH) cycle is at the heart of redox metabolism, linking the major redox buffers with central metabolism through the processing of reactive oxygen species (ROS) and pyridine nucleotide metabolism. Tomato fruit development is underpinned by changes in redox buffer contents and their associated enzyme capacities, but interactions between them remain unclear. Based on quantitative data obtained for the core redox metabolism, we built an enzyme‐based kinetic model to calculate redox metabolite concentrations with their corresponding fluxes and control coefficients. Dynamic and associated regulations of the ASC–GSH cycle throughout the whole fruit development were analysed and pointed to a sequential metabolic control of redox fluxes by ASC synthesis, NAD(P)H and ROS availability depending on the developmental phase. Furthermore, we highlighted that monodehydroascorbate reductase and the availability of reducing power were found to be the main regulators of the redox state of ASC and GSH during fruit growth under optimal conditions. Our kinetic modelling approach indicated that tomato fruit development displayed growth phase‐dependent redox metabolism linked with central metabolism via pyridine nucleotides and H2O2 availability, while providing a new tool to the scientific community to investigate redox metabolism in fruits.

Publisher

Wiley

Subject

Plant Science,Physiology

Cited by 8 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3