Cas12a‐based on‐site, rapid detection of genetically modified crops

Author:

Duan Zhiqiang1,Yang Xiaoliang2,Ji Xingkun1,Chen Ying3,Niu Xiaomu3,Guo Anping2,Zhu Jian‐Kang456,Li Feng3,Lang Zhaobo7,Zhao Hui2

Affiliation:

1. Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off‐Season Reproduction Regions Institute of Tropical Bioscience and Biotechnology & San Ya Research Institute, Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China

2. Bellagen Biotechnology Co. Ltd. Jinan 250000 China

3. College of Life Sciences, Shandong Normal University Jinan 250014 China

4. Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and Technology Shenzhen 518055 China

5. Center for Advanced Bioindustry Technologies Chinese Academy of Agricultural Sciences Beijing 100081 China

6. Hainan Yazhou Bay Seed Lab Sanya Hainan 572024 China

7. Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences Shanghai 201602 China

Publisher

Wiley

Subject

Plant Science,General Biochemistry, Genetics and Molecular Biology,Biochemistry

Reference8 articles.

1. International Service for the Acquisition of Agri‐biotech Applications (ISAAA). (2019). ISAAA Brief 55–2019: Executive summary: Biotech crops drive socio‐economic development and sustainable environment in the new frontier.

2. CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

3. Current and new approaches in GMO detection: Challenges and solutions;Fraiture M.A.;BioMed. Res. Int.,2015

4. Cas12a trans‐cleavage can be modulated in vitro and is active on ssDNA, dsDNA, and RNA;Fuchs R.T.;BioRxiv,2019

5. Nucleic acid detection with CRISPR-Cas13a/C2c2

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