Affiliation:
1. Hubei Key Laboratory of Natural Products Research and Development, College of Chemistry and Life Science, China Three Gorges University, Yichang, China
Abstract
Abstract
Objectives
Reactive oxygen species can induce cell apoptosis, and oxidative stress has been implicated in a variety of neurodegenerative disorders. Tomatine, which is a naturally occurring steroidal glycoalkaloid isolated from Solanum cathayanum, has shown potent anti-oxidant properties.
Methods
In this study, we used the SH-SY5Y cell line as an in vitro model and investigated the protective effect of tomatine against hydrogen peroxide (H2O2)-induced neurotoxicity in SH-SY5Y cells.
Key findings
Tomatine might inhibit the release of cellular lactate dehydrogenase, increase anti-oxidant enzyme activity and glutathione content, reverse the downregulated protein expression of the brain-derived neurotrophic factor (BDNF), inhibit expression of Bax and activations of caspase-3 and caspase-9 in H2O2-induced SH-SY5Y cells.
Conclusions
Tomatine exerted beneficially neuroprotective effect on H2O2-induced SH-SY5Y cells, mainly enhancing intracellular anti-oxidant enzyme activity and BDNF expression, inhibiting H2O2-induced oxidative stress as well as expression of Bax and activations of caspase-3 and caspase-9, alleviating H2O2-induced SH-SY5Y cell injury and cell death.
Funder
National Natural Science Foundation of China
Publisher
Oxford University Press (OUP)
Subject
Pharmaceutical Science,Pharmacology
Cited by
24 articles.
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