Highly specific qPCR and amplicon sequencing method for detection of quarantine citrus pathogen Phyllosticta citricarpa applicable for air samples

Author:

Zajc Janja12ORCID,Kogej Zwitter Zala13ORCID,Fišer Sara1,Gostinčar Cene4ORCID,Vicent Antonio5ORCID,Domenech Anaïs Galvañ5ORCID,Riccioni Luca6ORCID,Boonham Neil7ORCID,Ravnikar Maja1ORCID,Kogovšek Polona1ORCID

Affiliation:

1. Department of Biotechnology and Systems Biology National Institute of Biology Ljubljana Slovenia

2. Agricultural Institute of Slovenia Ljubljana Slovenia

3. Jožef Stefan International Postgraduate School Ljubljana Slovenia

4. Biotechnical Faculty, Department of Biology University of Ljubljana Ljubljana Slovenia

5. Institut Valencià d'Investigacions Agràries (IVIA), Centre de Protecció Vegetal i Biotecnologia Moncada Valencia Spain

6. Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca difesa e certificazione (CREA‐DC) Rome Italy

7. School of Natural and Environmental Sciences Newcastle University Newcastle upon Tyne UK

Funder

European Food Safety Authority

European Social Fund

Javna Agencija za Raziskovalno Dejavnost RS

Publisher

Wiley

Subject

Horticulture,Plant Science,Genetics,Agronomy and Crop Science

Reference52 articles.

1. Combining permanent aerobiological networks and molecular analyses for large‐scale surveillance of forest fungal pathogens: A proof‐of‐concept

2. Regulation (EU) 2016/2031 of the European parliament of the Council of 26 October 2016 on protective measures against pests of plants, amending Regulations (EU) No 228/2013, (EU) No 652/2014 and (EU) No 1143/2014 of the European Parliament and of the Council and repealing Council Directives 69/464/EEC, 74/647/EEC, 93/85/EEC, 98/57/EC, 2000/29/EC, 2006/91/EC and 2007/33/EC;Anon;Official Journal of the European Union,2016

3. Regulation (EU) 2019/2072 of 28 November 2019 establishing uniform conditions for the implementation of regulation (EU) 2016/2031 of the European Parliament and the council, as regards protective measures against pests of plants, and repealing commission regulation (EC) No 690/2008 and amending commission implementing regulation (EU) 2018/2019;Anon;Official Journal of the European Union,2019

4. A fungal mock community control for amplicon sequencing experiments

5. DNA metabarcoding uncovers fungal diversity of mixed airborne samples in Italy

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