Novel insights into orchid mycorrhiza functioning from stable isotope signatures of fungal pelotons

Abstract

Summary Stable isotope signatures of fungal sporocarps have been instrumental in identifying carbon gains of chlorophyllous orchids from a fungal source. Yet, not all mycorrhizal fungi produce macroscopic sporocarps and frequently fungi of different taxa occur in parallel in orchid roots. To overcome this obstacle, we investigated stable isotope signatures of fungal pelotons extracted from orchid roots and compared these data to the respective orchid and reference plant tissues. Anoectochilus sandvicensis and Epipactis palustris represented specialized or unspecialized rhizoctonia‐associated orchids. Epipactis atrorubens and Epipactis leptochila are orchids considered ectomycorrhiza‐associated with different preferences for Basidio‐ and Ascomycota. 13C enrichment of rhizoctonia pelotons was minor compared with plant tissues and significantly lower than enrichments of pelotons from ectomycorrhizal Epipactis species. 15N values of pelotons from E. leptochila and E. atrorubens showed similar patterns as known for respective sporocarps of ectomycorrhizal Ascomycota and Basidiomycota, however, with an offset towards lower 15N enrichments and nitrogen concentrations. Our results suggest an explicit fungal nutrition source of orchids associated with ectomycorrhizal fungi, whereas the low 13C enrichment in rhizoctonia‐associated orchids and fungal pelotons hamper the detection of carbon gains from fungal partners. 15N isotopic pattern of orchids further suggests a selective transfer of 15N‐enriched protein‐nitrogen into orchids.