Diurnal changes in apoplast bypass flow of water and ions in salt‐stressed wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.)

Abstract

AbstractThe aim of the present study was to quantify the contribution of apoplastic bypass flow to the uptake of water and salt across the root cylinder of wheat and barley during day and night. Plants were grown on hydroponics until they were 14–17 days old and then analysed over a single day (16 h) or night (8 h) period while being exposed to different concentrations of NaCl (50, 100, 150 and 200 mM NaCl). Exposure to salt started just before the experiment (short‐term stress) or had started 6d before (longer‐term stress). Bypass flow was quantified using the apoplastic tracer dye 8‐hydroxy‐1,3,6‐pyrenesulphonic acid (PTS). The percent contribution of bypass flow to root water uptake increased in response to salt stress and during the night and amounted to up to 4.4%. Bypass flow across the root cylinder of Na+ and Cl− made up 2%–12% of the net delivery of these ions to the shoot; this percentage changed little (wheat) or decreased (barley) during the night. Changes in the contribution of bypass flow to the net uptake of water, Na+ and Cl− in response to salt stress and day/night are the combined result of changes in xylem tension, the contribution of alternative cell‐to‐cell flow path and the requirement to generate xylem osmotic pressure.