Histopathological characterisation of trunk‐dominant canine pemphigus foliaceus, and comparison with classic facial and insecticide‐triggered forms

Abstract

AbstractBackgroundWhile the clinical features were described recently, the histopathological characterisation of trunk‐dominant canine pemphigus foliaceus (PF) is lacking, and whether it differs from classic facial or insecticide‐triggered PF is unknown.Hypothesis/ObjectivesThis study describes the histopathological findings of trunk‐dominant PF, and compares the results to classic facial and insecticide‐triggered PF.AnimalsSkin biopsies from 103 dogs with clinically characterised trunk‐dominant (n = 33), classic facial (n = 26) and insecticide‐triggered PF (n = 44) were included.Materials and MethodsHistological sections, randomised and blinded, were scored for over 50 morphological parameters of pustules, epidermis, dermis, adnexa and crusts. Intact pustule area and width were measured by digital microscopy.ResultsIn trunk‐dominant PF, 77 intact pustules were predominantly subcorneal (0.0019–1.940 mm2 area, 0.0470–4.2532 mm wide), and contained from one to over 100 acantholytic keratinocytes. Pustules had boat acantholytic cells, corneocytes, perinuclear eosinophilic rings, neutrophil rosettes, acantholytic cell necrosis, rafts, cling‐ons and/or eosinophils. Peripustular epidermal spongiosis, necrosis and lymphocyte exocytosis occurred, as did follicular pustules. Mixed dermal inflammation often contained eosinophils. Trunk‐dominant PF did not differ from the other PF groups except for few parameters, such as having fewer rafts (p = 0.003). Additional autoimmune inflammatory patterns occurred in all PF groups.Conclusions and Clinical RelevanceTrunk‐dominant PF and other canine PF variants are histologically similar, which indicates shared pathomechanisms. The identification of common boat acantholytic cells and corneocyte separation has implications for the mechanisms of acantholysis. The diversity of histopathological and polyautoimmunity features support complicated immune mechanisms. Finally, results indicate that diagnostic biopsies cannot differentiate between these PF variants in dogs.