Affiliation:
1. Key Laboratory of Tea Science of Ministry of Education College of Horticulture, Hunan Agricultural University Changsha 410128 China
2. Hainan Yazhou Bay Seed Laboratory Sanya Nanfan Research Institute of Hainan University Sanya 572025 China
3. State Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University Hefei 230036 China
4. College of Horticulture Sichuan Agricultural University Chengdu 611130 China
5. Department of Plant and Soil Sciences Institute for Agricultural Biosciences, Oklahoma State University 3210 Sam Noble Parkway Ardmore OK 73401 USA
Abstract
SUMMARYAnthocyanin and catechin production in tea (Camellia sinensis) leaves can positively affect tea quality; however, their regulatory mechanisms are not fully understood. Here we report that, while the CsMYB75‐ or CsMYB86‐directed MYB–bHLH–WD40 (MBW) complexes differentially activate anthocyanin or catechin biosynthesis in tea leaves, respectively, CsMYBL2a and CsMYBL2b homologs negatively modified the light‐ and temperature‐induced anthocyanin and catechin production in both Arabidopsis and tea plants. The MBW complexes activated both anthocyanin synthesis genes and the downstream repressor genes CsMYBL2a and CsMYBL2b. Overexpression of CsMYBL2b, but not CsMYBL2a, repressed Arabidopsis leaf anthocyanin accumulation and seed coat proanthocyanin production. CsMYBL2b strongly and CsMYBL2a weakly repressed the activating effects of CsMYB75/CsMYB86 on CsDFR and CsANS, due to their different EAR and TLLLFR domains and interactions with CsTT8/CsGL3, interfering with the functions of activating MBW complexes. CsMYBL2b and CsMYBL2a in tea leaves play different roles in fine‐tuning CsMYB75/CsMYB86–MBW activation of biosynthesis of anthocyanins and catechins, respectively. The CsbZIP1–CsmiR858a–CsMYBL2 module mediated the UV‐B‐ or cold‐activated CsMYB75/CsMYB86 regulation of anthocyanin/catechin biosynthesis by repressing CsMYBL2a and CsMYBL2b. Similarly, the CsCOP1–CsbZIP1–CsPIF3 module, and BR signaling as well, mediated the high temperature repression of anthocyanin and catechin biosynthesis through differentially upregulating CsMYBL2b and CsMYBL2a, respectively. The present study provides new insights into the complex regulatory networks in environmental stress‐modified flavonoid production in tea plant leaves.
Funder
National Natural Science Foundation of China
Subject
Cell Biology,Plant Science,Genetics
Cited by
5 articles.
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