Differential Response of MYB Transcription Factor Gene Transcripts to Circadian Rhythm in Tea Plants (Camellia sinensis)

Author:

Hu Zhihang12ORCID,Zhang Nan2,Qin Zhiyuan1,Li Jinwen1,Yang Ni1,Chen Yi1,Kong Jieyu1,Luo Wei1,Xiong Aisheng2ORCID,Zhuang Jing1ORCID

Affiliation:

1. Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

2. State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China

Abstract

The circadian clock refers to the formation of a certain rule in the long-term evolution of an organism, which is an invisible ‘clock’ in the body of an organism. As one of the largest TF families in higher plants, the MYB transcription factor is involved in plant growth and development. MYB is also inextricably correlated with the circadian rhythm. In this study, the transcriptome data of the tea plant ‘Baiyeyihao’ were measured at a photoperiod interval of 4 h (24 h). A total of 25,306 unigenes were obtained, including 14,615 unigenes that were annotated across 20 functional categories within the GO classification. Additionally, 10,443 single-gene clusters were annotated to 11 sublevels of metabolic pathways using KEGG. Based on the results of gene annotation and differential gene transcript analysis, 22 genes encoding MYB transcription factors were identified. The G10 group in the phylogenetic tree had 13 members, of which 5 were related to the circadian rhythm, accounting for 39%. The G1, G2, G8, G9, G15, G16, G18, G19, G20, G21 and G23 groups had no members associated with the circadian rhythm. Among the 22 differentially expressed MYB transcription factors, 3 members of LHY, RVE1 and RVE8 were core circadian rhythm genes belonging to the G10, G12 and G10 groups, respectively. Real-time fluorescence quantitative PCR was used to detect and validate the expression of the gene transcripts encoding MYB transcription factors associated with the circadian rhythm.

Funder

National Natural Science Foundation of China

Provincial Policy Guidance Program North Jiangsu Science and Technology Special Project

Collection and Creation of Horticultural Crop Germplasm Resources of Jiangsu

Priority Academic Program Development of Jiangsu Higher Education Institutions Project

Publisher

MDPI AG

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