<scp>AAV</scp>‐mediated <i>Gpm6b</i> expression supports hair cell reprogramming-Reference-Cited by-同舟云学术

AAV‐mediated Gpm6b expression supports hair cell reprogramming

Published:2024-02-24 Issue:7 Volume:57 Page:
ISSN:0960-7722
Container-title:Cell Proliferation
language:en
Short-container-title:Cell Proliferation

Author:

Sun Qiuhan¹,Zhang Liyan¹,Chen Tian¹,Li Nianci¹,Tan Fangzhi¹,Gu Xingliang¹,Zhou Yinyi¹,Zhang Ziyu¹,Lu Yicheng¹,Lu Jie²,Qian Xiaoyun³,Guan Bing²,Qi Jieyu¹,Ye Fanglei⁴,Chai Renjie¹⁵⁶⁷⁸^ORCID

Affiliation:

1. State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High‐Tech Key Laboratory for Bio‐Medical Research Southeast University Nanjing China

2. Northern Jiangsu People's Hospital Affiliated to Yangzhou University/Clinical Medical College Yangzhou University Yangzhou China

3. Department of Otolaryngology‐Head and Neck Surgery, the Affiliated Drum Tower Hospital of Nanjing University Medical School Jiangsu Provincial Key Medical Discipline(Laboratory) Nanjing China

4. Department of Otology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China

5. Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu China

6. Co‐Innovation Center of Neuroregeneration Nantong University Nantong China

7. Institute for Stem Cells and Regeneration Chinese Academy of Science Beijing China

8. Southeast University Shenzhen Research Institute Shenzhen China

Abstract

AbstractIrreversible damage to hair cells (HCs) in the cochlea leads to hearing loss. Cochlear supporting cells (SCs) in the murine cochlea have the potential to differentiate into HCs. Neuron membrane glycoprotein M6B (Gpm6b) as a four‐transmembrane protein is a potential regulator of HC regeneration according to our previous research. In this study, we found that AAV‐ie‐mediated Gpm6b overexpression promoted SC‐derived organoid expansion. Enhanced Gpm6b prevented the normal decrease in SC plasticity as the cochlea develops by supporting cells re‐entry cell cycle and facilitating the SC‐to‐HC transformation. Also, overexpression of Gpm6b in the organ of Corti through the round window membrane injection facilitated the trans‐differentiation of Lgr5+ SCs into HCs. In conclusion, our results suggest that Gpm6b overexpression promotes HC regeneration and highlights a promising target for hearing repair using the inner ear stem cells combined with AAV.

Funder

Science and Technology Department of Sichuan Province

Fundamental Research Funds for the Central Universities

National Key Research and Development Program of China

National Natural Science Foundation of China

Publisher

Wiley

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/cpr.13620

Reference44 articles.

1. Gene therapy for the inner ear

2. Gene therapy for hair cell regeneration: Review and new data

3. Challenges in Cell-Based Therapies for the Treatment of Hearing Loss

4. Hair Cell Regeneration

5. Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells

Cited by 3 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A human-specific cytotoxic neopeptide generated by the deafness gene Cingulin;Journal of Genetics and Genomics;2024-08

2. Recent Progress in Generation of Inner Ear Organoid;Advanced Biology;2024-07-25

3. In Utero Gene Therapy and its Application in Genetic Hearing Loss;Advanced Biology;2024-07-15