Kinetics of neutrophil extracellular traps and cytokines in oral mucositis and Candida infection

Author:

de Arruda José Alcides Almeida1ORCID,Oliveira Sicília Rezende1ORCID,Heimlich Fernanda Vieira1ORCID,de Amorim‐Santos Bárbara Maria2ORCID,Schneider Ayda Henriques3ORCID,de Sena Ana Carolina Velasco Pondé4ORCID,Rodrigues Karla Emília de Sá5ORCID,Macari Soraia6ORCID,Souza Daniele Glória2ORCID,Travassos Denise Vieira78,Abreu Lucas Guimarães9ORCID,Silva Tarcília Aparecida17ORCID,Mesquita Ricardo Alves1ORCID

Affiliation:

1. Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil

2. Department of Microbiology, Institute of Biological Sciences Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil

3. Department of Pharmacology, Ribeirão Preto Medical School Universidade de São Paulo Ribeirão Preto São Paulo Brazil

4. Postgraduate Program in Dentistry and Health, School of Dentistry Universidade Federal da Bahia Salvador Bahia Brazil

5. Department of Pediatric Oncology Children’s Cancer Hospital, Barretos Cancer Hospital Barretos São Paulo Brazil

6. Department of Restorative Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil

7. Multiprofessional Integrated Residency in Health, Hospital das Clínicas Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil

8. Department of Social and Preventive Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil

9. Department of Child and Adolescent Oral Health, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil

Abstract

AbstractObjectiveThis study investigated the concentrations of neutrophil extracellular traps (NET) and salivary cytokines (IL‐1β, IL‐6, IL‐8/CXCL8, TNF, and TGF‐β1) in patients undergoing chemotherapy and their associations with oral mucositis (OM) and Candida infection.Materials and MethodsThis prospective longitudinal study performed at a Brazilian service included 60 adults diagnosed with hematolymphoid diseases. Saliva samples were collected on days D0, D3, D10, and D15. Cytokines were analyzed by ELISA and NET formation by identification of the myeloperoxidase‐DNA complex. Oral Candida spp. was cultured.ResultsOM occurred in 43.3% of patients and oral candidiasis in 20%. However, 66% of individuals had positive cultures for C. albicans. Higher concentrations of IL‐6, IL‐8/CXCL8, and TNF and lower concentrations of TGF‐β1 were observed in patients with OM. C. albicans infection contributed to the increase in IL‐8/CXCL8, TGF‐β1, and TNF. Individuals with OM or with oral candidiasis had significant reductions in NET formation. In contrast, individuals with C. albicans and with concomitant C. albicans and OM exhibited higher NET formation.ConclusionThe kinetics of cytokine levels and NET formation in chemotherapy‐induced OM appears to be altered by Candida infection, even in the absence of clinical signs of oral candidiasis.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Wiley

Subject

General Dentistry,Otorhinolaryngology

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