Bio Efficacy Assay of Laccase Isolated and Characterized from Trichoderma viride in Biodegradation of Low Density Polyethylene (LDPE) and Textile Industrial Effluent Dyes

Abstract

This study is based on the biosynthesis of laccase enzyme from fungi Trichoderma viride and its exploitation in biodegradation of polyethylene using Laccase mediator system (LMS -Laccase + 1-HBT) in Low Density Polyethylene (LDPE) and in biodegradation of textile industrial effluent dyes. In different parts of our lifespan we have seen the numerous types of plastics are getting degraded by various methods, still the average time to completely degrade a plastic bottle is at least 450 years.Similarly industry produces over 3.6 thousand individual textile dyes today and utilizes more than 8000 toxic chemicals in numerous methods for textile manufacture comprising dyeing and printing. As polyethylene and textile industrial effluent dyes are causing severe hazardous effect on environment and health issues in all kind of living organisms, it is necessary to degrade plastics and textile industrial effluent dyes in rapid way. Recently researchers have come up with an idea of degrading plastic and textile industrial effluent dyes with the help of microorganisms and enzymes much faster than normal rate. The Laccase enzyme extracted was tested for its optimum temperature and pH. Lowry’s method is used for protein estimation. A control and sample LDPE was subjected to LMS. The tensile strength and elongation of the sample was less than that of the control after 5 days of treatment. This study showed that laccase together with 1-HBT helps to biodegrade polyethylene. The purified laccase enzyme was used for the pretreatment assay and post treatment assay. The Laccase degrades certain reactive dyes like Congo red, Acid Red, Methylene Blue, Brilliant Blue, Metallic Blue and Black. Thus recommends the application of laccase in textile dye colour removal (bioremediation).