Optimization of Laccase Enzyme Production by Amesia atrobrunnea A2: A First Report

Abstract

ABSTRACT: Lignin is one of the recalcitrant compounds largely present in enormous amount on earth. It is considered as major paper industries pollutant because of its brown color and hydrophobicity. Laccase is one of the major lignin degrading enzymes for solution of recent environmental problem. A study was carried out on fungi to establish laccase production potential for industrial and environmental application. The present research for laccase activity was reported by screening 16 isolates from rotted wood samples and agro waste collected from Patan, Gujarat. One of the isolated fungal species showing highest production of laccase enzyme activity was identified to be Amesia atrobrunnea A2. The objective of this work was to isolate laccase producing fungal isolate and optimize the production of laccases by Amesia atrobrunnea A2 in submerged fermentation. Among six variables (temperature, pH, carbon, nitrogen, inducer and cation sources), glucose as carbon source and veratryl alcohol as inducer were identified as good enhancer of laccase production.