The effect of proteolytic enzymes on the amino acid composition of the filtrate and the grain mash obtained from the shock activator disintegrating treated rye

Abstract

The effect of enzymes of proteolytic action on the amino acid composition of the filtrate and grains of grain wort obtained from UDA-treated rye was studied. The feasibility of using a complex of proteolytic enzymes: neutral and acid proteases is shown. The processing of rye was carried out on the disintegrator DEZI15 with different working elements: three and five-row rotors rotating at a frequency of 120 s1. A control sample of grinding was obtained in a laboratory mill with a rotor-knife working body. According to the data obtained on the Malvern Mastersizer 2000 laser analyzer, the average particle size in the grinding samples was: on a disintegrator with a three-row rotor – 167.5 microns, on a five-row rotor-knife working body – 384.278 microns. As a source of proteolytic enzymes, preparations of the fungal protease Рrо100L and the bacterial protease Protosubtilin GZх A120 were used. The determination of the content of ?-amino nitrogen in the finished wort (mg / dm3) was performed by calorimetry with ninhydrin. The concentration of amino acids was determined using the KNAUER amino acid analyzer: the calculation of aminograms was carried out by comparing the areas of the standard and the sample. It was established that the UDA-treatment allows obtaining a high content of ?-amino nitrogen in the filtrate of rye hydrolysates, and the complex introduction of acidic and neural proteases strengthens this effect. Analysis of the amino acid composition of the filtrate and grains showed the presence of all the essential amino acids in both of these products. The filtrate can be recommended as a nutrient nitrogen-containing medium for the cultivation of microorganisms, followed by the release of secondary metabolites of their vital activity, or as an intermediate in the production of sugar-containing syrups from whole grains with a high content of amino acids. The fraction of the hydrolyzate is of interest as a protein-containing feed additive, and the fine particle size distribution of such a product will allow it to be used not only for feeding cattle, but also in poultry farming.