Genomic relationships among sixteen species of Avena based on (ACT)6 trinucleotide repeat FISH

Author:

Luo Xiaomei1,Tinker Nick A.2,Zhou Yonghong3,Wight Charlene P.2,Liu Juncheng1,Wan Wenlin1,Chen Liang1,Peng Yuanying3

Affiliation:

1. College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, Sichuan Province, China.

2. Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, KW Neatby Bldg., Central Experimental Farm, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada.

3. Triticeae Research Institute, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, Sichuan Province, China.

Abstract

Knowledge of the locations of repeat elements could be very important in the assembly of genome sequences and their assignment to physical chromosomes. Genomic and species relationships among 16 species were investigated using fluorescence in situ hybridization (FISH) with the Am1 and (ACT)6 probes. The Am1 oligonucleotide probe was particularly enriched in the C genomes, whereas the (ACT)6 trinucleotide repeat probe showed a diverse distribution of hybridization patterns in the A, AB, C, AC, and ACD genomes but might not be present in the B and D genomes. The hybridization pattern of Avena sativa was very similar to that of A. insularis, indicating that this species most likely originated from A. insularis as a tetraploid ancestor. Although the two FISH probes failed to identify relationships of more species, this proof-of-concept approach opens the way to the use of FISH probes in assigning other signature elements from genomic sequence to physical chromosomes.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,General Medicine,Biotechnology

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