Rapid detection of Clostridium perfringens enterotoxin by a modified ligated intestinal loop technique in rabbits

Abstract

The ligated intestinal loop technique in rabbits for the assay of Clostridium perfringens enterotoxin was improved by decreasing the incubation time from about 6 h to 90 min, and keeping the animal under anaesthesia during this period.The fluid volumes were significantly higher in loops injected with enterotoxin than in loops injected either with saline or with a mixture of enterotoxin and specific antiserum. These differences were mainly due to a net loss of fluid from the control loops.