Affiliation:
1. Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, China.
2. College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Abstract
A Texel sheep ear marginal tissue fibroblast cell line (named TSF19) was successfully established by using a primary explant technique and cell cryoconservation technology. TSF19 cells were adherent, with a population doubling time of 24.9 h. Chromosome analysis showed that >90% of cells were diploid prior to cell passage 4. Isoenzyme analyses of lactate dehydrogenase and malate dehydrogenase showed that the TSF19 cells had no cross-contamination with other species. Tests for cell line contamination with bacteria, fungi, or mycoplasmas were also negative. Plasmids encoding the fluorescent proteins pEGFP-N3, pECFP-N1, pDsRed1-N1, and pEYFP-N1 were transfected into cells to study exogenous gene expression in the cells. The plasmid transfection efficiency was between 21.8% and 46.5%. This newly established cell line will not only preserve the genetic resources of the important Texel sheep at the cell level but will also provide a valuable resource for genomic, postgenomic, somatic cloning research.
Publisher
Canadian Science Publishing
Subject
Cell Biology,Molecular Biology,Biochemistry
Reference34 articles.
1. Doyle, A., Hay, R., and Kirsop, B.E. eds. 1990. Animal cells, living resources for biotechnology. Cambridge University Press, Cambridge, U.K. pp. 81–100.
2. Critical assessment of the nuclear import of plasmid during cationic lipid-mediated gene transfer
3. Freshney, R.I. ed. 1992. Animal cell culture: a practical approach. Oxford University Press, Oxford, U.K. pp. 119–122.
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