Affiliation:
1. Department of Biological Sciences, Centennial Centre for Interdisciplinary Science, University of Alberta, Edmonton, AB T6G 2E9, Canada.
2. Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.
Abstract
Cereal crop plants have low nitrogen (N) use efficiency, taking up only 30% to 50% of the applied N fertilizers, with the rest having the potential for loss into the environment as N pollution. One way to address this problem is to improve the nitrogen use efficiency of cereal crops using a transgenic approach. We developed alanine aminotransferase overexpressing rice, and we have previously determined that this modification provided an improved nitrogen-use phenotype to the engineered plants. In this study, the transgenic rice were grown in low, medium, and high nitrogen supply, and morphology, plant N levels, enzymatic activity, metabolite levels, and transcriptome response in the roots and shoots at active and maximum tillering at each N level were measured. The transcriptome response was analysed further using MapMan and PageMan to view multiple comparisons. The transgenic rice plants showed improved nitrogen use efficiency at medium and high N supply, but with few significant changes to the amino acid levels or to the transcriptome. The transgenic plants grown in high N showed up-regulation of transcripts associated with photosynthesis, non-melavonate pathway secondary metabolites, protein degradation, and many unknown function transcripts.
Publisher
Canadian Science Publishing
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Cited by
35 articles.
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