GLYCYL-RNA SYNTHETASE OF RAT LIVER: PARTIAL PURIFICATION AND EFFECTS OF SOME METAL IONS ON ITS ACTIVITY-Reference-Cited by-同舟云学术

GLYCYL-RNA SYNTHETASE OF RAT LIVER: PARTIAL PURIFICATION AND EFFECTS OF SOME METAL IONS ON ITS ACTIVITY

Published:1963-01-01 Issue:1 Volume:41 Page:1123-1133
ISSN:0576-5544
Container-title:Canadian Journal of Biochemistry and Physiology
language:en
Short-container-title:Can. J. Biochem. Physiol.

Author:

Fraser M. J.¹

Affiliation:

1. The Biochemistry Department, University of Manitoba Medical School, Winnipeg, Manitoba

Abstract

Glycyl-RNA synthetase has been purified 40-fold from a 105,000 × g supernatant of an homogenate of rat liver by successive precipitation at pH = 5.0, heat treatment at 55 °C for 3.0 minutes in the presence of 1.0 mM ATP, and ammonium sulphate fractionation. The purified fractions catalyzed glycine-dependent ATP-32PP exchange. The effect of some metal ions on glycine activation was studied. Activation occurred in the presence of either Mg++or Mn++. The apparent stimulation of glycine activation by Co++was found to be an artifact.

Publisher

Canadian Science Publishing

Subject

General Medicine

Link

http://www.nrcresearchpress.com/doi/pdf/10.1139/y63-128

Reference21 articles.

1. Chairman's Introduction: Some Facts and Problems

2. Some Problems Concerning the Activation of Amino Acids

3. Observations on amino acid-dependent exchanges of inorganic pyrophosphate and ATP

4. Steps in amino-acid incorporation into mammary tissue

5. The carboxyl activation of glycine in extracts of photobacterium fischeri

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. ACETYLATION OF GLYCYL-RNA;Canadian Journal of Biochemistry;1965-07-01

2. SOME PROPERTIES OF RAT LIVER GLYCYL-RNA SYNTHETASE;Canadian Journal of Biochemistry;1964-12-01