Author:
Gutierrez Noemi A.,Maddox Ian S.
Abstract
An investigation was made into some methods of culture maintenance and inoculum development procedures by which the production of solvents by Clostridium acetobutylicum could be maximized. Storage of the organism as spores in nonnutrient media at either 4 or −20 °C proved to be the most effective method of culture maintenance. On revival of stock cultures, neither heat shocking nor treatment with aqueous ethanol or butanol gave improvements in solvent production during the subsequent fermentation. However, significantly improved production was obtained when culture transfers during the inoculum development procedure were made at the time of maximum cell motility.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
10 articles.
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