Interactive effects of temperature and salinity on otolith chemistry: challenges for determining environmental histories of fish

Abstract

The concentrations of elements and isotopes in fish otoliths may provide a method of reconstructing movements of fish by differentiating between water bodies of different temperatures and salinities. However, before otoliths can be used to reconstruct environmental histories of fish, it is necessary to assess the effects of seawater temperature and salinity on otolith microchemistry. Using controlled laboratory experiments, juvenile black bream, Acanthopagrus butcheri (family Sparidae), were reared for 50 days in aquaria of varying temperatures and salinities using three experimental designs: temperature × salinity, temperature only, and salinity only. Temperature and salinity interacted to significantly affect the elemental concentration ratios of Sr:Ca and Ba:Ca and the concentrations of isotopes δ13C and δ18O in otoliths. The single-factor experiments showed that temperature significantly affected the concentration ratios of Sr:Ca and Ba:Ca and the concentrations of δ13C and δ18O in otoliths, whereas salinity alone did not affect the concentration ratios of any elements but did affect both isotopes. The concentration ratios of Mg:Ca and Mn:Ca varied considerably among fish within the same treatment level and showed little or no effects due to temperature and (or) salinity. The significant interactive effects of temperature and salinity on otolith microchemistry highlight the need for a multifactorial approach to testing hypotheses regarding the environmental histories of fish.