Metabolic regulation of endoglucanase synthesis in Trichoderma reesei: participation of cyclic AMP and glucose-6-phosphate

Abstract

The synthesis of endoglucanase by young induced mycelia of Trichoderma reesei QM 9414 incubated in the presence of 1 mM sophorose (a potent cellulase inducer) was stimulated or repressed by additions of dibutyryl cyclic AMP (dBcAMP), depending on the concentration. At low concentrations (10−6 and 10−5 M), dBcAMP stimulated the formation of endoglucanase; higher concentrations of dBcAMP (10−3 and 10−2 M) repressed the synthesis of endoglucanase. Addition of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) at 1 and 10 μM concentrations to young induced mycelia caused an increase in intracellular cAMP and stimulated the production of endoglucanase. Neither exogenous dBcAMP nor IBMX was capable of inducing endoglucanase synthesis by itself, and neither was able to relieve catabolite repression of endoglucanase synthesis caused by glucose. All of the monosaccharides tested caused a more or less transient increase in intracellular cAMP. However, the effect of these treatments on endoglucanase synthesis was varied. The phosphorylable hexoses, both metabolizable and nonmetabolizable, increased the intracellular level of glucose-6-phosphate or its analogs and repressed endoglucanase synthesis. Nonphosphorylable sugars, such as 6-deoxyglucose, xylose, L-fucose, and (or) L-sorbose, did not influence the glucose-6-phosphate level and stimulated endoglucanase production to varying degrees. It is concluded that both cAMP and glucose-6-phosphate are involved in regulating cellulase synthesis in T. reesei. However, these factors seem to act in opposing directions.Key words: endoglucanase, induction, catabolite repression, metabolic regulation, glucose-6-phosphate, cyclic AMP, Trichoderma reesei.