Phosphonic acid analogues of carbohydrate metabolites. Dihydroxypropylphosphonic acid. II. Substrate specificity of L-glycerol-3-phosphate : NAD oxidoreductase

Abstract

The specificity of L-glycerol-3-phosphate : NAD oxidoreductase with regard to the phosphate ester bond of L-α-glycerophosphoric acid was investigated by means of L- and DL-dihydroxypropylphosphonic acid. The phosphate ester bond was found to be an essential structural requirement for enzyme activity. Neither L- nor DL-dihydroxypropylphosphonic acid has an inhibitory effect on the oxidation of L-α-glycerophosphoric acid by L-glycerol-3-phosphate : NAD oxidoreductase.