Disc electrophoretic comparisons of proteins and peroxidases from Phaseolus vulgaris leaves infected with Agrobacterium tumefaciens

Abstract

Soluble proteins extracted from Phaseolus vulgaris primary leaves infected with Agrobacterium tumefaciens were separated by disc electrophoresis and stained with Coomassie blue. Wounded but noninfected healthy leaves were used as a control. Gel scans revealed little difference in protein profiles between healthy and tumored leaves. Peroxidase isoenzymes were visualized on the gels with benzidine dihydrochloride – H2O2. Distinct differences were found in the number and activity of peroxidase bands between extracts. Extracts from tumored leaves contained more intensely stained peroxidase isoenzymes than healthy leaves. Leaves that were inoculated with a non-tumor-producing strain of A. tumefaciens had essentially the same protein and peroxidase profile as the healthy leaves.