Genetic association of crown rust resistance genePc68, storage protein loci, and resistance gene analogues in oats

Author:

Satheeskumar Sivakala1,Sharp Peter J.1,Lagudah Evans S.2,McIntosh Robert A.1,Molnar Stephen J.3

Affiliation:

1. The University of Sydney, Plant Breeding Institute, 107 Cobbitty Road, Cobbitty NSW 2570, Australia.

2. CSIRO Plant Industry, GPO Box 1600, Canberra ACT 2601, Australia.

3. Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, K.W. Neatby Building, Central Experimental Farm, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada.

Abstract

Segregating F3families, derived from a cross between oat cultivar Swan and the putative single gene line PC68, were used to determine the association of seed storage protein loci and resistance gene analogues (RGAs) with the crown rust resistance gene Pc68. SDS–PAGE analysis detected three avenin loci, AveX, AveY, and AveZ, closely linked to Pc68. Their diagnostic alleles are linked in coupling to Pc68 and were also detected in three additional lines carrying Pc68. Another protein locus was linked in repulsion to Pc68. In complementary studies, three wheat RGA clones (W2, W4, and W10) detected restriction fragment length polymorphisms (RFLPs) between homozygous resistant and homozygous susceptible F3DNA bulks. Four oat homologues of W2 were cloned and sequenced. RFLPs detected with two of them were mapped using F3and F4populations. Clone 18 detected a locus, Orga2, linked in repulsion to Pc68. Clone 22 detected several RFLPs including Orga1 (the closest locus to Pc68) and three RGA loci (Orga22-2, Orga22-3, and Orga22-4) loosely linked to Pc68. The diagnostic RFLPs linked in coupling to Pc68 were detected by clone 22 in three additional oat lines carrying Pc68 and have potential utility in investigating and improving crown rust resistance of oat.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,General Medicine,Biotechnology

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