Mitosis in the yeast phase of Agaricostilbum pulcherrimum and its evolutionary significance

Abstract

Agaricostilbum pulcherrimum is an anomaly and is difficult to place systematically. It possesses a yeast phase, and as in most basidiomycetous yeasts, mitosis has not been investigated cytoiogically. Yeast cells of A. pulcherrimum were prepared for immunofluorescence and transmission electron microscopy by a freeze-substitution method. A cladistic analysis of cell cycle characters among A. pulcherrimum and two ascomycetous and two basidiomycetous yeasts, performed with phylogenetic analysis using parsimony, revealed that A. pulcherrimum is basal within these basidiomycetes. Spindle pole bodies are multilayered discs and appear to be intranuclear during early division, similar to meiotic division. Spindle initiation and early elongation occur in the parent, a situation unreported in basidiomycetous yeasts. The site of spindle initiation, the position of the nucleus during division, and the pattern of astral microtubules demonstrate that the mode of nuclear division in A. pulcherrimum is intermediate between those of the investigated ascomycetous and basidiomycetous yeasts. Keywords: basidiomycete, cell cycle, cytoskeleton, immunofluorescence, phylogeny, spindle pole body.