Inheritance and linkage relationships of some isozymes of black spruce in New Brunswick

Abstract

Thirteen polymorphic loci from eleven enzyme systems in black spruce (Piceamariana (Mill.) B.S.P.) were studied by gel electrophoresis using embryos and haploid megagametophyte tissue. Two loci (Mdh-1 and Sdh) showed deviations from the expected 1:1 segregation ratio of megagametophytes from heterozygous maternal plants that were significant at the 1 % level and a third (Aat) at the 5% level. Of the 78 possible pairwise comparisons for the linkage analysis, 58 could be tested for jointly independent segregation. Linkage could be detected between three pairs of loci: Aat–Pgi, Gdh–Idh, and Fum–Pgm.