Segregation and Linkage Relationships of Allozymes in Pinus Brutia Ten-Reference-Cited by-同舟云学术

Segregation and Linkage Relationships of Allozymes in Pinus Brutia Ten

Published:2006-12-01 Issue:1-6 Volume:55 Page:155-160
ISSN:2509-8934
Container-title:Silvae Genetica
language:en
Short-container-title:

Author:

Kaya Nuray¹,Isik K.¹

Affiliation:

1. Akdeniz University, Faculty of Arts and Sciences, Department of Biology, 07058 Antalya , Turkey

Abstract

Abstract Female gametophytes of Pinus brutia Ten. were analyzed to study Mendelian segregation and linkage among allozyme loci in a seed orchard composed of 28 clones. Isozyme variants of nine enzyme systems encoding 14 loci from megagametophytes were assayed by starch gel electrophoresis. While six of the 14 loci were monomorphic, the remaining eight were polymorphic. Analysis of observed segregation ratios of all polymorphic loci except for Sdh1, showed a good fit to the 1:1 ratios expected for Mendelian inheritance. Linkage relationships of the eight polymorphic allozyme loci were analyzed by testing 23 of the 28 possible two-locus combinations. Two-locus segregations in three pairs (Got2- Mdh1, Got2-Pgi2 and Pgd3-Sdh1) were significantly heterogeneous (P ≤ 0.05). Relatively weak linkage was observed between only one pair of genes (Adh2:Got2). Recombination fraction for Adh2:Got2 was found to be 0.24 (± 0.06).

Publisher

Walter de Gruyter GmbH

Subject

Genetics,Forestry

Reference18 articles.

1. ADAMS, W. T. and R. J. JOLY (1980): Linkage relationships among twelve enzyme loci in loblolly pine. The Journal of Heredity 71: 199-202.

2. ADAMS, W. T., D. B. NEALE, A. H. DOERKSEN and D. B. SMITH (1990): Inheritance and linkage of isozyme variants from seed and vegetative bud tissues in coastal Douglas-fir [Pseudotsuga menziesii var. menziesii (Mirb.) Franco]. Silvae Genetica 39: 153-167.

3. BOYLE, T. J. and E. K. MORGENSTERN (1985): Inheritance and linkage relationships of some isozymes of black spruce in New Brunswick. Can. J. For. Res. 15: 992-996.

4. BROWN, A. H. D., A. C. MATHESON and K. G. ELDRIDGE (1975): Estimation of the mating system of Eucalyptus obliqua L’Herit. by using allozyme polymorphisms. Aust. J. Bot. 28: 931-949.

5. CHELIAK, W. M., K. MORGAN, B. P. DANCIK, C. STROBECK and F. C. H. YEH (1984): Segregation of allozymes in megagametophytes of viable seed from a natural population of jack pine, Pinus banksiana Lamb. Theor. Appl. Genet. 69: 145-151.