Author:
Dvorak J.,Harvey B. L.,Coulman B. E.
Abstract
The use of N2O as a polyploidizing agent in barley and wheat proved to be effective in both species when applied in the interval from 4 to 24 hr after pollination in barley and 24 to 48 hr after pollination in wheat. When treated in these intervals with four atmospheres pressure, 75% of the barley and 54% of the wheat embryos were polyploid. There was a marked difference between the two species in the proportion of aneuploids produced. In barley 5% of the treated embryos were aneuploid whereas 42% of the wheat embryos were aneuploid in the critical treatments.Evidence is presented to show that most of the aneuploids originated from disturbances in the anaphase distribution of chromosomes or from a scattering of chromosomes before the formation of restitution nuclei rather than from multipolar divisions.A potential use of N2O for producing homozygous diploid plants of Hordeum vulgare L. emend Lam. from crosses between Hordeum vulgare and H. bulbosum L. is discussed.
Publisher
Canadian Science Publishing
Subject
Cell Biology,Plant Science,Genetics
Cited by
28 articles.
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