Quantitative determination of urea concentrations in cell culture medium-Reference-Cited by-同舟云学术

Quantitative determination of urea concentrations in cell culture medium

Published:2009-06 Issue:3 Volume:87 Page:541-544
ISSN:0829-8211
Container-title:Biochemistry and Cell Biology
language:en
Short-container-title:Biochem. Cell Biol.

Author:

Zawada Robert J.X.¹²,Kwan Peggy¹²,Olszewski Kellen L.¹²,Llinas Manuel¹²,Huang Shu-Gui¹²

Affiliation:

1. BioAssay Systems LLC, 3423 Investment Blvd., Suite 11, Hayward, CA 94545, USA.

2. Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics, Carl Icahn Laboratory, Princeton University, Princeton, NJ 08540, USA.

Abstract

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.

Publisher

Canadian Science Publishing

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

http://www.nrcresearchpress.com/doi/pdf/10.1139/O09-011

Reference10 articles.

1. New Colorimetric Reaction for End-Point, Continuous-Flow, and Kinetic Measurement of Urea

2. Enzymic urea assay: a new colorimetric method based on hydrogen peroxide measurement.

3. Kinetic assay of serum and urine for urea with use of urease and leucine dehydrogenase

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