Effect of carbon and nitrogen nutrition on growth and sporulation of Trichoderma viride Pers. ex Fries

Abstract

Three isolates of Trichoderma viride Pers. ex Fries were grown in liquid cultures. The isolates grew best on media containing glucose, sucrose, cellobiose, and mannitol.Asparagine and ammonium nitrate proved to be the best sources of nitrogen for the three isolates although isolate 1503 did not show any significant preferences for the five sources of nitrogen. The amounts of carbon and nitrogen in the basal medium significantly influenced the growth of the three isolates. However, the addition of asparagine alone did not produce a better growth than in the control. There is no correlation between sporulation and dry weight of culture, the amounts of carbon in the medium, and C/N ratio. Implications of the findings are briefly discussed.