A STUDY OF CERTAIN FACTORS INFLUENCING SPORULATION OF SACCHAROMYCES CEREVISIAE

Abstract

A decline in sporulation noted with cells from cultures aged 7–30 days was not due to a decrease in the proportion of viable cells. The optimum cell population density for sporulation in acetate solution was two to four millions per milliliter and seemed unaffected by acetate concentration. Cells from three presporulation media that allowed moderate growth sporulated poorly in acetate solution. Seven assimilable sugars stimulated sporulation at low concentration in water agar, while three non-assimilable sugars and mannitol did not. Glucose-1-phosphate, fructose-1,6-diphosphate, and sodium aspartate were stimulating. The following were non-stimulating: sodium formate, sodium propionate, propionic acid, sodium oxalate, oxalic acid, sodium citrate, citric acid, aminoacctic acid, glutaric acid, indole-3-acetic acid, acetaldehydc, ethyl alcohol, histamine, glucosamine. Differences are pointed out between the chemical conditions favoring growth and sporulation.