Cnida sequestration in aeolid nudibranchs: variability and retention time of sequestered cnidae in the opalescent sea slug, Hermissenda crassicornis (Gastropoda, Nudibranchia)

Abstract

Aeolid sea slugs can isolate and store stinging cnidae obtained from their cnidarian prey, presumably for their own defence. There are 30 different varieties of cnidae, identified by their unique structure. The aeolids engulf the cnidae and store them in a functional state at the tips of their cerata. Although the process of cnida sequestration is reasonably well understood in aeolids, two critical questions remain: (1) are cnida types uniformly distributed among the cerata and (2) how long do sequestered cnidae persist? I collected opalescent sea slugs (Hermissenda crassicornis (Eschscholtz, 1831)) from Barkley Sound, British Columbia, Canada, and determined the cnida complements in four cerata per individual by microscope. The cnida complements differed between cerata from different body regions within the individual (values of Whittaker’s dissimilarity index from 2.5% to 36.3%). Furthermore, the cnidae varieties in low abundances are not consistently present within an individual. I also found that H. crassicornis fed a non-cnidarian diet lost cnidae over time, but the cnidarian-fed individuals did not: 3 of 10 H. crassicornis on the non-cnidarian diet lacked cnidae completely at 42 days. Future studies of cnida sequestration should be mindful that one ceras does not give an adequate representation of the distribution of sequestered cnidae.