Diazinon disrupts antipredator and homing behaviors in chinook salmon (Oncorhynchus tshawytscha)

Abstract

Neurotoxic pesticides are known to contaminate surface waters that provide habitat for salmonids, including some listed for protection under the U.S. Endangered Species Act. Despite their widespread use, the impacts of these pesticides on the neurological health of wild salmon are not well understood. Of particular concern are the organophosphate and carbamate insecticides that block synaptic transmission by inhibiting neuronal acetylcholinesterase. Here we assess the effects of diazinon, an organophosphate insecticide, on alarm pheromone induced antipredator responses and homing behavior in chinook salmon (Oncorhynchus tshawytscha). Nominal exposure concentrations (0.1, 1.0, and 10.0 µg·L-1) were chosen to emulate diazinon pulses in the natural environment. In the antipredator study, diazinon had no effect on swimming behavior or visually guided food capture. However, the pesticide significantly inhibited olfactory-mediated alarm responses at concentrations as low as 1.0 µg·L-1. Similarly, homing behavior was impaired at 10.0 µg·L-1. Our results suggest that olfactory-mediated behaviors are sensitive to anticholinesterase neurotoxicity in salmonids and that short-term, sublethal exposures to these insecticides may cause significant behavioral deficits. Such deficits may have negative consequences for survival and reproductive success in these fish.