DETERMINATION OF VOLATILE ACID PRODUCTION OF CLOSTRIDIUM BY GAS CHROMATOGRAPHY

Author:

Lewis Vester J.,Moss C. Wayne,Jones Wallis L.

Abstract

Gas–liquid chromatography was used to determine volatile fatty acid production by pure cultures of 15 species of Clostridium. Acetic acid was detected from each of the 39 strains examined, although only in trace amounts from C. bulyricum. Butyric acid was detected in considerable amounts from all organisms except the three strains of C. sporogenes tested. In addition, several species produced one or more of the following acids: valeric, isovaleric, isobutyric, and isocaproic. The technique of gas–liquid chromatography proved to be both reliable and rapid for the characterization of volatile acids produced by the bacteria under the experimental conditions employed.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology

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