Characterization of two LuxI/R homologs in Pantoea ananatis LMG 2665T-Reference-Cited by-同舟云学术

Characterization of two LuxI/R homologs in Pantoea ananatis LMG 2665T

Published:2016-11 Issue:11 Volume:62 Page:893-903
ISSN:0008-4166
Container-title:Canadian Journal of Microbiology
language:en
Short-container-title:Can. J. Microbiol.

Author:

Sibanda Siphathele¹²,Theron Jacques¹,Shyntum Divine Y.¹,Moleleki Lucy N.¹²,Coutinho Teresa A.¹²

Affiliation:

1. Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, Republic of South Africa.

2. Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, Republic of South Africa.

Abstract

Quorum sensing (QS) plays an important role in the regulation of bacteria–host interactions and ecological fitness in many bacteria. In this study, 2 luxI/R homologs, namely eanI/eanR and rhlI/rhlR, were identified in the genome sequence of Pantoea ananatis LMG 2665T. To determine a role for these luxI/R homologs in pathogenicity and biofilm formation, mutant bacterial strains lacking either eanI/R or rhlI/R and both of these homologs were generated. The results indicated that both the RhlI/R and EanI/R systems are required for pathogenicity and biofilm formation in strain LMG 2665T. This is the first study to characterize the biological significance of the RhlI/R QS system in P. ananatis.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology

Link

http://www.nrcresearchpress.com/doi/pdf/10.1139/cjm-2016-0143

Reference40 articles.

1. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

2. Detection of Quorum Sensing Signal Molecules and Identification of an Autoinducer Synthase Gene among Biofilm Forming Clinical Isolates of Acinetobacter spp.

3. Identification of the central quorum sensing regulator of virulence in the enteric phytopathogen, Erwinia carotovora: the VirR repressor

4. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant

5. Salivary Proteins of Lygus hesperus (Hemiptera: Miridae)

Cited by 9 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Pantoea ananatis , a plant growth stimulating bacterium, and its metabolites isolated from Hydrocotyle umbellata (dollarweed);Plant Signaling & Behavior;2024-03-22

2. Role of a Quorum Sensing Signal Acyl-Homoserine Lactone in a Phytobiome;Phytobiomes: Current Insights and Future Vistas;2020

3. Functional Characterization of a Global Virulence Regulator Hfq and Identification of Hfq-Dependent sRNAs in the Plant Pathogen Pantoea ananatis;Frontiers in Microbiology;2019-09-11

4. Quorum Sensing in Pseudomonas savastanoi pv. savastanoi and Erwinia toletana: Role in Virulence and Interspecies Interactions in the Olive Knot;Applied and Environmental Microbiology;2018-09-15

5. Quorum Sensing in Gram-Negative Plant Pathogenic Bacteria;Advances in Plant Pathology;2018-08-29