Salivary Proteins of Lygus hesperus (Hemiptera: Miridae)

Abstract

ABSTBACT Lygus hesperus Knight (Hemiptera: Miridae) causes economic damage to a wide range of crops in the western United States. While stylet-probing, L. hesperus discharge saliva consisting of lytic enzymes that facilitate extraoral digestion of host tissues. L. hesperus saliva primarily consists of polygalacturonases, α-amylases, and proteases, but salivary constituents other than these enzymes have not been documented. Improved knowledge of L. hesperus salivary proteins could lead to a better understanding of Lygus-host interactions, and aid in the development of crops that are resistant or tolerant to injury caused by Lygus spp. The objective of our study was to use mass spectrometry to identify salivary proteins of L. hesperus adults. Multiple polygalacturonases and proteases were identified from diet that was stylet-probed and fed upon by L. hesperus. Laccase, alkyl hydroperoxide reductase-like protein, glucose dehydrogenase, and xanthine dehydrogenase were identified in styletprobed diets by using mass spectrometry, and laccase activity was confirmed using a colorimetric assay. This is the first study to identify laccase, glucose dehydrogenase, and xanthine dehydrogenase from L. hesperus saliva; we propose these enzymes target plant-defense compounds. Nearly 100 proteins from the bacterial plant pathogen, Pantoea ananatis and nine proteins from Serratia spp. were identified in stylet-probed diets. These results suggest L. hesperus may vector these plant pathogens. Our study adds to the list of documented L. hesperus salivary proteins, and provides information that will be useful for the further study of L. hesperus saliva and Lygus-microbe interactions.