CD24 and CD49f expressions of E14.5 mouse mammary anlagen cells define putative distribution of earlier embryonic mammary stem cell activities

Author:

Song Jiazhe12,Ding Fangrong3,Li Song3,Li Wenzhe1,Li Ning23,Xue Kai1

Affiliation:

1. College of Basic Medical Sciences, Dalian Medical University, Dalian, P. R. China.

2. State Key Laboratories for AgroBiotechnology, College of Biological Sciences, China Agricultural University, Beijing, P. R. China.

3. National Animal Genetic Research Center, Beijing, P. R. China.

Abstract

Stem cell biology offers promise for understanding the origins of the mammary gland. However, the distribution of mammary stem cell (MaSC) activities at earlier embryonic stages has not been fully identified. The markers for sorting adult MaSC, CD24, CD29, and CD49f have been applied to analyze fetal MaSCs. Here we explored mammary anlagen MaSCs by investigating the expression of CD24 and CD49f. According to the comparative analysis between adult mammary gland and fetal mammary anlagen, we found that fetal mouse mammary anlagen may possess a high percentage of potential MaSCs. Flow cytometry analysis revealed 2 distinct mammary anlagen populations: LinCD24med and LinCD24high. Sphere-forming and mammary repopulating assays confirmed that the stem cell activity of E14.5 mouse mammary anlagen was restricted to the LinCD24med cell population. Furthermore, CD24med mammary anlagen cells were separated into LinCD24medCD49f+ and LinCD24medCD49f populations and identified, respectively. The results proved that the mammary anlagen LinCD24medCD49f+ cell population possesses more stem cell activities than the LinCD24medCD49f cell population. However, a limited numbers of stem cells and large numbers of stromal cells were identified in mammary anlagen in the LinCD24med cell population.

Publisher

Canadian Science Publishing

Subject

Cell Biology,Molecular Biology,Biochemistry

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