A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper

Abstract

The hypersensitive resistance to tomato spotted wilt virus (TSWV) in pepper is determined by a single dominant gene (resistant allele: Tsw) in several Capsicum chinense genotypes. In order to facilitate the selection for this resistance, four RAPD (among 250 10-mer primers tested) were found linked to the Tsw locus using the bulked segregant analysis and 153 F2individuals. A close RAPD marker was converted into a codominant cleaved amplified polymorphic sequence (CAPS) using specific PCR primers and restriction enzymes. This CAPS marker is tightly linked to Tsw (0.9 ± 0.6 cM) and is helpful for marker-assisted selection in a wide range of genetic intercrosses. Key words: Capsicum sp., PCR marker, bulked segregant analysis, Tospovirus, resistance gene.