Author:
Krotoski W. A.,Benjamin D. C.,Weimer H. E.
Abstract
The effects of starch concentration on the resolution of serum components by gel electrophoresis were investigated. Gels were prepared with starch contents varying from 11.8 to 20.6% (w/v). Pooled serum samples from man, rabbit, guinea pig, rat, mouse, and chicken were separated by vertical and two-dimensional electrophoresis in the discontinuous Tris–citrate and borate system of buffers. The resolution of serum components at the various starch levels was compared with the results obtained with the standard concentration (11.8%) recommended for separation in human serum. A general increase in the number of bands staining for protein was observed in gels with starch concentrations between 13.0 and 16.0%, although species differences were observed in regard to optimum starch concentrations. The maximum number of serum proteins detected by one-dimensional electrophoresis were, for the various species: man, 27; rabbit, 15; guinea pig, 22; rat, 21; mouse, 19; and chicken, 12.
Publisher
Canadian Science Publishing
Cited by
6 articles.
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