REDUCTION AND ALKYLATION OF HUMAN PLASMA OROSOMUCOID IN AQUEOUS SOLUTION AND IN 8 MUREA: A PHYSICOCHEMICAL AND IMMUNOCHEMICAL STUDY: HUMAN OROSOMUCOID: REDUCTION AND ALKYLATION

Author:

Krotoski W. A.,Weimer H. E.

Abstract

Highly purified orosomucoid (α1-acid glycoprotein) was exposed to mercaptoethanol, iodoacetamide and 8 M urea, in various combinations. The derivatives were examined by agar-gel electrophoresis, by acrylamide-gel "disc" electrophoresis (employing an improved method), by immunodiffusion techniques, and by quantitative immunochemical methods.The results indicated an —S—S— dependent tertiary structure for the polypeptide backbone, but one which could not be separated into subunits by the methods employed. The mechanisms of reduction and alkylation of the glycoprotein are discussed, and several alternate structural models for the polypeptide moiety are presented.

Publisher

Canadian Science Publishing

Subject

General Medicine

Cited by 10 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Electrophoretic and immunoelectrophoretic study of the enzymatic degradation of human orosomucoid (α1 -acid glycoprotein);International Journal of Peptide and Protein Research;2009-01-12

2. Photochemical degradation of C.I. acid black 1;Dyes and Pigments;1992-01

3. The denaturation of orosomucoid;Archives of Biochemistry and Biophysics;1982-07

4. Isolation and characterization of a hemagglutinin from Amphitrite ornata, a polychaetous annelid;Biochimica et Biophysica Acta (BBA) - Protein Structure;1976-08

5. α1-Acid Glycoprotein;The Plasma Proteins;1975

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