Effects of cytokinin and abscisic acid on heat resistance of Vetiveria zizanioides

Abstract

Vetiveria zizanioides is ideal in maintaining soil and water, and is widely used for remediation of soil contaminated by heavy metals. However, it is affected by high-temperature stress. In this study, Vetiveria zizanioides plants were sprayed with 6-BA and ABA in a growth chamber 1 d before heat stress treatment, then the plants were subjected to high-temperature conditions. Relative water content, relative electrical conductivity, contents of ascorbic acid (AsA) and reduced glutathione (GSH) as the antioxidative substances, and content of malondiadehyde (MDA) were determined. Also, the antioxidative enzyme activities and the osmoprotectants levels were detected. Diaminobenzidine (DAB) staining of leaves and roots in Vetiveria zizanioides was observed for determination of hydrogen peroxide accumulation. The results showed that relative water content was decreased, relative electrical conductivity and MDA content were increased by the heat stress treatment. Under high-temperature conditions, relative water content was increased and relative electrical conductivity was decreased by 6-BA and ABA treatments. At the middle and the late stages of the heat stress treatment, activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and the contents of ASA and GSH in leaves of the 6-BA-treated and ABA-treated plants were significantly higher, whereas the MDA content was significantly lower than those in the high-temperature controls. Hydrogen peroxide accumulation levels in the 6-BA-treated and ABA-treated leaves and roots were lower than in the high-temperature controls. Contents of soluble sugar, sucrose, and glucose in the 6-BA-treated and ABA-treated leaves were significantly higher than those in the high-temperature controls. Proline content in the 6-BA-treated and ABA-treated leaves was stable. The results suggested that the enhancement of the heat resistance by 6-BA and ABA treatments was correlated with the activation of the antioxidant system, as well as the sugar-based osmoprotectant.