Author:
FREITAS Rodrigo Therezan de,PAIVA Renato,SALES Thais Silva,SILVA Diogo Pedrosa Corrêa da,REIS Michele ValquÃria dos,SOUZA Ana Cristina de,ROSA Sttela Dellyzete Veiga Franco da
Abstract
As a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ‘Catuaà Vermelho’ - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption.
Publisher
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Subject
Horticulture,Plant Science,Agronomy and Crop Science
Cited by
7 articles.
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