Efficient Approaches to in vitro Multiplication of Lilium candidum L. with Consistent and Safe Access throughout Year and Acclimatization of Plant under Hot-Summer Mediterranean (Csa Type) Climate

Abstract

Lilium candidum L. is one of the most fragrant plant species among Liliaceae. The indigenous and natural populations of L. candidum are significantly affected by fast increasing anthropological population pressure and increased carbon fuel pollution. There is a necessity for development of a micropropagation protocol to serve in effective manner for both commercial multiplication and protection of the plant. The study targeted to develop a strategy for effective in vitro plant propagation system using static liquid culture, seismomorphogenic treatments with mixed liquid culture and semi solid culture medium using single scale explants. The former two techniques failed to regenerate new bulblets effectively whereby the later technique induced 100% regeneration on all treatments. Maximum number of bulblets regenerated on 0.27 µM Thidiazuron + 1.08 µM Naphthalene acetic acid. The scale explant could be recultured and regenerated on the mother culture medium for six cycles. The daughter bulblets regenerated on Murashige and Skoog medium containing both 0.18 or 0.27 µM TDZ + 1.08 µM NAA were successfully rooted using 1/2 × MS medium containing 2.45 µM indole butyric acid. Regardless of rooting development, acclimatization was noted on all bulblets in the greenhouse. These plants are showing continuous flowering since last four years under hot-summer Mediterranean (CSa type) climate field conditions of Tekirdag province of Thrace region in Turkey.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********