New immunoassay systems based on recombinant human lactoferrin

Author:

Semenov D. A.1,Vashkevich I. I.1,Sviridov O. V.1

Affiliation:

1. Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus

Abstract

In this work, soluble and solid phase immunoreagents, including recombinant human lactoferrin (rhLF), a complex of rhLF with europium ions, rabbit antiserum to rhLF, anti-rhLF immunoglobulin purified by antigen-affinity chromatography and the conjugates of this immunoglobulin with an Eu3+ chelate or horseradish peroxidase have been obtained by a combination of biochemical and synthetic methods using rhLF as an initial compound. Biospecific interactions of the reagents in four immunochemical systems were assessed by measuring the enzyme activity or time-resolved fluorescence. The study resulted in the development of fast and precise immunoassays for biologically active rhLF in transgenic goat milk and in protein fractions obtained in the course of pure rhLF manufacture, as well as in pharmaceutical preparations and food additives.

Publisher

Publishing House Belorusskaya Nauka

Reference17 articles.

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Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Interaction of recombinant human lactoferrin and SARS-CoV-2 virus to heparin-protein conjugate;Doklady of the National Academy of Sciences of Belarus;2022-09-08

2. Some metal binding properties of recombinant human lactoferrin from the milk of transgenic goats;Doklady of the National Academy of Sciences of Belarus;2022-03-09

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