Affiliation:
1. Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Abstract
In this research, the ability of pure recombinant human lactoferrin (rhLF), originated from the milk of transgenic goats, to bind ferric and europium ions has been shown by the methods of spectrophotometry, fluorescent spectroscopy, and inductively coupled plasma mass spectrometry. The apo-form of rhLF and its complexes with Fe3+ or Eu3+ saturated in 76 or 22 %, respectively, were obtained. A method for detection of total or released (“free”) lanthanide at acidic or neutral pH and high or low concentrations of chelating agents by time-resolved fluorescence was proposed.
Publisher
Publishing House Belorusskaya Nauka
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