Evaluating differences in optical properties of indolent and aggressive murine breast tumors using quantitative diffuse reflectance spectroscopy

Author:

Rodriguez Troncoso Joel1,Marium Mim Umme1,Ivers Jesse D.1,Paidi Santosh K.2ORCID,Harper Mason G.1,Nguyen Khue G.1,Ravindranathan Sruthi1,Rebello Lisa1,Lee David E.1,Zaharoff David A.3,Barman Ishan2,Rajaram Narasimhan14ORCID

Affiliation:

1. University of Arkansas

2. Johns Hopkins University

3. , University of North Carolina and North Carolina State University

4. University of Arkansas for Medical Sciences

Abstract

We used diffuse reflectance spectroscopy to quantify tissue absorption and scattering-based parameters in similarly sized tumors derived from a panel of four isogenic murine breast cancer cell lines (4T1, 4T07, 168FARN, 67NR) that are each capable of accomplishing different steps of the invasion-metastasis cascade. We found lower tissue scattering, increased hemoglobin concentration, and lower vascular oxygenation in indolent 67NR tumors incapable of metastasis compared with aggressive 4T1 tumors capable of metastasis. Supervised learning statistical approaches were able to accurately differentiate between tumor groups and classify tumors according to their ability to accomplish each step of the invasion-metastasis cascade. We investigated whether the inhibition of metastasis-promoting genes in the highly metastatic 4T1 tumors resulted in measurable optical changes that made these tumors similar to the indolent 67NR tumors. These results demonstrate the potential of diffuse reflectance spectroscopy to noninvasively evaluate tumor biology and discriminate between indolent and aggressive tumors.

Funder

University of Arkansas for Medical Sciences

National Institute of General Medical Sciences

National Cancer Institute

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Biotechnology

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