Label-free study on the effect of a bioactive constituent on glioma cells in vitro using terahertz ATR spectroscopy

Author:

Liao Yunsheng12,Zhang Mingkun13,Tang Mingjie13,Chen Ligang13,Li Xueqin2,Liu Zhongdong2,Wang Huabin13ORCID

Affiliation:

1. Chongqing Institute of Green and Intelligent Technology

2. Henan University of Technology

3. University of Chinese Academy of Sciences

Abstract

In this work, we report that the effect of bioactive constituent on living glioma cells can be evaluated using terahertz time-domain attenuated total reflection (THz TD-ATR) spectroscopy in a label-free, non-invasive, and fast manner. The measured THz absorption coefficient of human glioma cells (U87) in cell culture media increases with ginsenoside Rg3 (G-Rg3) concentration in the range from 0 to 50 µM, which can be interpreted as that G-Rg3 deteriorated the cellular state. This is supported either by the cell growth inhibition rate measured using a conventional cell viability test kit or by the cellular morphological changes observed with fluorescence microscopy. These results verify the effectiveness of using the THz TD-ATR spectroscopy to detect the action of G-Rg3 on glioma cells in vitro. The demonstrated technique thus opens a new route to assessing the efficacy of bioactive constituents on cells or helping screen cell-targeted drugs.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

the University of Chinese Academy of Sciences Supported Program for Tackling Key Problems in Science and Technology

Natural Science Foundation of Chongqing

Beibei District Science and Technology Agency Supported Program for Science and Technology Talents & Independent Innovation

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Biotechnology

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